Manitoba Integrated Cell Purification and Analysis Unit

Institution: 
The Institute of Molecular Biology and Genetics
DK Zabolotny Institute of Microbiology and Virology
Address: 
415 Apotex Centre, 750 McDermot Avenue Winnipeg, MB R3E 0T5
Lab/facility website: 
http://www.umanitobaflow.ca/index.html
Published date: 
Dec 26, 2015
What the lab/facility does: 
Research on immunology, allergy and asthma, infectious disease and cancer
Sectors of Application: 
one
two
Area(s) of expertise: 
The Manitoba Integrated Cell Purification and Analysis Unit is a regionally unique state-of-the-art fluorescent cell purification, imaging and analysis facility with capabilities for specific isolation and analysis of live cell populations from human blood or other potentially infectious sources. The lab supports innovative research programs in immunology, allergy and asthma, infectious disease, cancer cell biology and stem cell biology.
Competed researched contracts: 
Flow cytometry-based cell purification (including rare cell types), fluorescence-based cellular analyses
Selected publications: 
Flow cytometry-based cell purification (including rare cell types), fluorescence-based cellular analyses
Research Services: 
Flow cytometry-based cell purification (including rare cell types), fluorescence-based cellular analyses
Specialized labs and equipment: 
Flow cytometry-based cell purification (including rare cell types), fluorescence-based cellular analyses

Name of specialized lab

Name of equipment

Description of function

Manitoba Integrated Cell Purification and Analysis Unit

FACS Aria 2 cell sorter

 

Sorting of cells based on multiple cell surface markers labeled with up to 12 fluorochromes, high-speed sorting for purification of sufficient numbers of rare cell populations, sorting of live cells from potentially infectious samples classified as biohazard level 2

 

Live cell spinning disk confocal microscope, with laser ablation/activation and TIRF imaging capabilities

High speed dynamic analysis of fluorescence labels in live cells

 

FACS Canto II cell analyzer

Analysis of cells based on multiple cell surface markers labeled with up to 8 fluorochromes